Journal:

Article Title: An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

doi:

Figure Lengend Snippet: (A) Southern blot analysis of plasmid preparations from spectinomycin-resistant transformants probed with plasmid pDL278. Lane 1, pDL278: lane 2, S. salivarius ATCC 25975; lane 3, L. lactis LM 0230; lane 4, P. acidilactici SMQ-250; lane 5, S. sanguis ATCC 10556; lane 6, S. sobrinus 6715; lane 7, S. vestibularis ATCC 49124; lane 8, S. thermophilus ATCC 19258; lane 9, S. thermophilus DT1 (now known as S. thermophilus SMQ-310); lane 10, S. salivarius ATCC 25975 Spr Cmr. (B) Southern blot analysis of plasmid preparations from chloramphenicol-resistant transformants probed with plasmid pNZ123. Lane 1, pNZ123; lane 2, S. salivarius ATCC 25975; lane 3, L. lactis LM 0230; lane 4, S. vestibularis ATCC 49124; lane 5, S. mutans XS123; lane 6, S. thermophilus DT1 (now known as S. thermophilus SMQ-310); lane 7, S. salivarius ATCC 25975 Spr Cmr.

Article Snippet: The sizes of the recovered plasmids corresponded to the original sizes of the transforming plasmids, indicating that the plasmids had not undergone any overt size modification following transformation into and replication in the new host. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Bacterium Transformation efficiency (transformants/μg of DNA) a pNZ123 pDL278 S. salivarius ATCC 25975 1.7 × 10 5 2.8 × 10 3 S. sanguis ATCC 10556 0 1.0 × 10 3 S. sobrinus 6715 0 39 S. thermophilus SMQ-310 8.8 × 10 3 2.6 × 10 3 S. thermophilus ATCC 19258 19 23 S. vestibularis ATCC 49124 1.7 × 10 5 8.4 × 10 4 S. mutans XS123 0 4.6 × 10 3 L. lactis LM 0230 3.7 × 10 4 9.3 × 10 3 P. acidilactici SMQ-250 0 112 Open in a separate window a All cells were transformed with 1 μg of plasmid DNA.

Techniques: Southern Blot, Plasmid Preparation

Journal:

Article Title: An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

doi:

Figure Lengend Snippet: Efficiencies of transformation of glycine-treated streptococci and related bacteria

Article Snippet: The sizes of the recovered plasmids corresponded to the original sizes of the transforming plasmids, indicating that the plasmids had not undergone any overt size modification following transformation into and replication in the new host. table ft1 table-wrap mode="anchored" t5 TABLE 5 caption a7 Bacterium Transformation efficiency (transformants/μg of DNA) a pNZ123 pDL278 S. salivarius ATCC 25975 1.7 × 10 5 2.8 × 10 3 S. sanguis ATCC 10556 0 1.0 × 10 3 S. sobrinus 6715 0 39 S. thermophilus SMQ-310 8.8 × 10 3 2.6 × 10 3 S. thermophilus ATCC 19258 19 23 S. vestibularis ATCC 49124 1.7 × 10 5 8.4 × 10 4 S. mutans XS123 0 4.6 × 10 3 L. lactis LM 0230 3.7 × 10 4 9.3 × 10 3 P. acidilactici SMQ-250 0 112 Open in a separate window a All cells were transformed with 1 μg of plasmid DNA.

Techniques: Transformation Assay

Journal:

Article Title: Coaggregation-Mediated Interactions of Streptococci and Actinomyces Detected in Initial Human Dental Plaque

doi: 10.1128/JB.185.11.3400-3409.2003

Figure Lengend Snippet: Indirect immunofluorescence reactions of streptococcal strains with anti-DL1 and anti-RPS

Article Snippet: Actinomyces spp. strains (Table ) whose names begin with N were isolated by L. V. H. Moore and W. E. C. Moore, and all strains in Table have been characterized according to the Moores' serotype scheme ( 11 ). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Species (biovar) a Strain b Reaction c with: Anti- RPS Anti- DL1 S. oralis SK92 , SK111 , 9811 , 34 + − ATCC 10557 , SK23 , SK143 , SK144 , C104 , 6249 − − S. mitis (1) SK137 + + J22 , 15914 + − SK141, SK142, H1 d − − S. mitis (2) SK96, SK148, K103, 903, H127 − + I18 − − S. gordonii (1) 38 + + SK6, SK120 , SK121 − + S. gordonii (2) SK184, DL1, e D105, K1, K4, M5, ATCC 10558, G102 − + S. gordonii (3) SK9, SK12 + + SK33, SK186 − + S. sanguinis (1) SK77 + + SK75 , SK72 + − SK1, SK36, SK37, ATCC 10556, SK76, SK85, SK108 − + S. sanguinis (2) SK4, SK156, SK157, SK158, SK164 − + S. sanguinis (3) SK163 + − SK150, SK159, SK160, SK162 − + S. sanguinis (4) SK45 , SK112 + − S. mutans ATCC33534, NCTC 10449 − − S. salivarius ATCC 9222 − + ATCC 25975 − − S. anginosus SK14, SK47, SK52, SK63, SK66, SK81, SK215, SK216, SK218, SK221 − − Open in a separate window a According to references 13 and 10 . b Strains in boldface are known to bear RPS ( 7 ). c Ten micrograms of primary antibody per ml; +, fluorescence equivalent to antigen strain fluorescence; −, no fluorescence or extremely dim. d Also known as S. oralis ATCC 55229, reclassified as S. mitis (biovar 1) ( 10 ). e Challis strain.

Techniques: Immunofluorescence

Journal:

Article Title: Galactose and Lactose Genes from the Galactose-Positive Bacterium Streptococcus salivarius and the Phylogenetically Related Galactose-Negative Bacterium Streptococcus thermophilus : Organization, Sequence, Transcription, and Activity of the gal Gene Products

doi: 10.1128/JB.184.3.785-793.2002

Figure Lengend Snippet: Strains and plasmids

Article Snippet: For the determination of sugar concentrations in the media of growing cells, samples (0.25 to 0.5 ml) were taken at intervals, heated at 100°C for 10 min, and centrifuged to remove cells and the supernatants were stored at −20°C until used for sugar assays. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant genotype or characteristics Source or reference Strains E. coli TOP10 F − mcrA Δ( mrr-hsdRMS-mcrBC ) φ80 lacZ ΔM15 Δ lacX74 deoR recA1 araD139 Δ( ara-leu7697 ) galU rpsL endAl nupG Invitrogen S. salivarius ATCC 25975 Wild type, Lac + Glu + Gal + 12 S. thermophilus SMQ-301 Wild type, Lac + Glu − Gal − 32 Plasmids pUC18 Cloning vector, Ap r lacZ ′ ( lacZ α) 37 pCR-Blunt Cloning vector Invitrogen PCR 2.1 TOPO Cloning vector, Ap r Ka r lacZ α Invitrogen pGAL1 Contains the last 1,040 nucleotides of galT and the first 526 nucleotides of galE from S. salivarius cloned into pCR-Blunt This work pGAL3 Contains the last 650 nucleotides of galK and the first 1,202 nucleotides of galT from S. salivarius cloned into pCR-Blunt This work pGAL29 Contains the first 882 nucleotides of galK and the first 853 nucleotides of galR from S. salivarius cloned into PCR 2.1 TOPO This work pGAL73 Contains the last 1,422 nucleotides of dexB , galR , and the first 729 nucleotides of galK from S. salivarius cloned into pUC18 This work pGAL123 Contains the last 104 nucleotides of galT , galE , and the first 928 nucleotides of galM from S. salivarius cloned into pUC18 This work Open in a separate window Strains and plasmids DNA purification and manipulations.

Techniques: Plasmid Preparation, Clone Assay

Journal:

Article Title: Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His~P) and HPr(Ser-P)(His~P) and Effects on Growth

doi: 10.1128/JB.185.23.6764-6772.2003

Figure Lengend Snippet: Strains and plasmids

Article Snippet: To determine sugar utilization by growing cells, cells were grown in tubes containing 10 ml of medium, and 0.2-ml samples were removed at intervals, heated at 100°C for 10 min to stop metabolism, centrifuged to remove cells, and stored at −20°C until sugar assays were conducted. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant genotype and/or characteristic(s) Source or reference Strains S. salivarius ATCC 25975 Wild-type, Lac + Glu + Gal + I. R. Hamilton, University of Manitoba G71 El-negative mutant derived from S. salivarius ATCC 25975 9 E. coli BL21 (DE3) F − ompT hsdS B (r B − m B − ) gal dcm ( DE3 ) Novagen XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q Z ΔM15 Tn 10 (Tet r )] Stratagene LMG194 F − Δ lacX74 galE thi rpsL Δ phoA ( Pvu II) Δ ara714 leu ::Tn 10 Invitrogen TOP10 F − mcrA Δ( mrr-hsdRMS-mcrBC ) φ 80lacZ Δ M15 Δ lacX74 deoR recA1 araD139 Δ( ara-leu ) 7697 galU rpsL endA1 nupG Invitrogen Plasmids pET-28a(+) Expression vector Novagen pET-29a(+) Expression vector Novagen pET-19b Expression vector Novagen pBAD/His Expression vector Invitrogen pCR-Blunt Cloning vector Invitrogen pETI-16 Contains the ptsI gene of S. salivarius cloned into pET-28a(+) This work pLacSIIA Contains the last 519 nucleotides of lacS from S. salivarius (which code for the IIA domain), cloned into pET29a(+) This work pLacSIIAH552R Contains the last 519 nucleotides of lacS from S. salivarius (which code for the IIA domain), with a mutation replacing LacS H552 by R, cloned into pET29a(+) This work pTML2 Contains the manL gene of S. salivarius cloned into pET19b This work pDR3 Contains the manH gene of S. salivarius cloned into pET19b This work pHPW18 Contains the ptsH gene of S. salivarius cloned into pBAD 8 pHPK229 Contains the hprK gene of S. salivarius cloned into pCR-Blunt 3 Open in a separate window Strains and plasmids

Techniques: Plasmid Preparation, Mutagenesis, Derivative Assay, Expressing, Clone Assay